PROBING HIDDEN POLYMERIC INTERFACES USINGIR–VISIBLE SUM-FREQUENCY GENERATION SPECTROSCOPY

Broad-Bandwidth Chiral Sum Frequency Generation Spectroscopy for Probing the Kinetics of Proteins at Interfaces

The kinetics of proteins at interfaces plays an important role in biological functions and inspires solutions to fundamental problems in biomedical sciences and engineering. Nonetheless, due to the lack of surface-specific and structural-sensitive biophysical techniques, it still remains challenging to probe protein kinetics in situ and in real time without the use of spectroscopic labels at in...

Investigation of Interfaces by Sum-Frequency Generation Vibrational Spectroscopy

Probing the Molecular Conformation of Self-Assembled Monolayers at Metal/Semiconductor Interfaces by Vibrational Sum Frequency Generation Spectroscopy

Theoretical Sum Frequency Generation Spectroscopy of Peptides

Vibrational sum frequency generation (SFG) has become a very promising technique for the study of proteins at interfaces, and it has been applied to important systems such as anti-microbial peptides, ion channel proteins, and human islet amyloid polypeptide. Moreover, so-called "chiral" SFG techniques, which rely on polarization combinations that generate strong signals primarily for chiral mol...

Chiral sum frequency generation for in situ probing proton exchange in antiparallel β-sheets at interfaces.

Studying hydrogen/deuterium (H/D) exchange in proteins can provide valuable insight on protein structure and dynamics. Several techniques are available for probing H/D exchange in the bulk solution, including NMR, mass spectroscopy, and Fourier transform infrared spectroscopy. However, probing H/D exchange at interfaces is challenging because it requires surface-selective methods. Here, we intr...